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e304 recombinant human ubch5a r d systems  (R&D Systems)


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    R&D Systems e304 recombinant human ubch5a r d systems
    E304 Recombinant Human Ubch5a R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e304+recombinant+human+ubch5a+r+d+systems/Recombinant+Human+UbcH5a%2FUBE2D1+Protein%2C+CF/pm39441718-622-144-148
    Average 95 stars, based on 83 article reviews
    e304 recombinant human ubch5a r d systems - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: EGF-mediated Golgi dynamics and cell migration require CARP2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GM130 Sigma Cat# G7295; RRID AB_532244 Anti-GFP Takara Cat# 632375; RRID:AB_2756343 Anti-Tubulin Sigma Cat# T6199 RRID:AB_477583 Anti-Actin Cell Signaling Technology Cat# 4967 RRID:AB_330288 Anti-GAPDH Abgenex Cat# 10-10011 Anti-Flag Sigma Cat# F3165-1MG; RRID:AB_259529 Anti-HA Sigma Cat# H6908 Anti-V5 Thermo Fisher Scientific Cat# R961-25, RRID:AB_2556565 Anti-BLZF1 Thermo Fisher Scientific Cat# MA5-27126, RRID:AB_2723990 Anti-GST Sigma Cat# G7781-25UL Anti-GORASP1 Elabscience Cat# E-AB-68390 Anti-GORASP2 Elabscience Cat# E-AB-63374 Anti-GOLGA2 Elabscience Cat# E-AB-11278 Anti-CARP2 Ravindran et al.40 N/A Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 61–6520, RRID:AB_2533933 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 65–6120, RRID:AB_2533967 Peroxidase AffiniPureTM Goat Anti-mouse IgG, light chain specific Jackson ImmunoResearch Cat# 115-035-174 RRID:AB_2338512 Peroxidase -conjugated monoclonal Mouse Anti-rabbit IgG, light chain specific Jackson ImmunoResearch Cat# 211-032-171 RRID:AB_2339149 Chemicals, peptides, and recombinant proteins Adenosine 5’-triphosphate magnesium salt Sigma Cat# A9187 Dulbecco’s modified Eagle’s medium Hyclone GE Cat# SH30243.01 Fetal Bovine Serum Gibco-Thermo Fisher Scientific Cat# 10270-106 Penicillin-streptomycin antibiotic solution HiMedia Cat# A001A Lipofectamine 3000 Invitrogen Cat# L3000-015 Lipofectamine RNAi Max Invitrogen Cat# 13778150 Epidermal Growth factor Sigma Cat# E9644 MitomycinC Sigma Cat# M4287 Nile red Sigma Cat# 72485 Albumin from bovine serum Sigma Cat# A9418 Acrylamide/Bis 29:1 (30%) Biorad Cat# 1610156 Bafilomycin A1 Merck Cat# 19-148 Biotin Sigma Cat# B4501-100MG 2-mercaptoethanol Sigma Cat# M6250 bromophenol blue HiMedia Cat # MB123 DAPI Sigma Cat# D8417 EGTA Sigma Cat# 03777-50G EDTA Sigma Cat# E5134-500G Glycerol Sigma Cat #G9012 Glutathione 4 Fast Flow GE Heathcare Cat# 17513201 Glass bottom dish Cellvis Plates Cat# D35-20-1.5-N Lysozyme Sigma Cat# 62970 Hoechst Stain Invitrogen Cat# 33342 (Continued on next page) Cell Reports 43, 114896, November 26, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER MG132 solution Sigma Cat# M7449-1ML MitoTracker Red CMXRos Invitrogen Cat# M7512 Ni-NTA His BIND resin Novagen Cat# 70666-4 Opti-MEM Reduced Serum Medium Gibco-Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Sigma Cat# 441244 Phosphatase inhibitor 2 Sigma Cat# P5726-1ML Phosphatase inhibitor 3 Sigma Cat# P0044-1ML Protease inhibitor cocktail Sigma Cat# P8340-1ML Protein G agarose beads Santacruz Cat# sc-2002 Protein A agarose beads Santacruz Cat# sc-2001 Prolong Gold Antifade reagent Invitrogen Cat# P36930 PVDF membrane Merck Cat# IPVH00010 Skim milk powder Himedia Cat# GRM1254-500G Sodium chloride Sigma Cat# S9888-5KG Sodium deoxycholate Sigma Cat# D6750-25G Sodium dodecyl sulfate Sigma Cat# L3771-1KG Sodium hydroxide Sigma Cat# 1.06498 Triton X-100 Sigma Cat# X100-5ML Trizma base Sigma Cat# T6066-5KG TRYPLE EXPRESS Invitrogen Cat# 12604021 Tween 20 Sigma Cat# P2287-500ML Immobilon Western Chemiluminescent HRP Substrate Merck Cat# WBKLS0500 Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1) R&D Systems Cat# E304 Recombinant Human UbcH5a R&D Systems Cat# E2-616 Experimental models: Cell lines HEK 293T ATCC N/A HEK293T vector control This paper N/A HEK293T CARP2 WT untagged This paper N/A HEK293T mRFP alone This paper N/A HEK293T CARP2WT-mRFP This paper N/A HeLa ATCC N/A .. N2A ATCC N/A A549 vector control Ravindran et al.40 N/A A549 CARP2 WT untagged Ravindran et al.40 N/A A549 CARP2 H333A untagged Ravindran et al.40 N/A A549 CARP2 C5A/C6A untagged Ravindran et al.40 N/A A549 CARP2 KO Ravindran et al.40 N/A A549 CARP2 KO Vector control Ravindran et al.40 N/A A549 CARP2 KO CARP2WT untagged Ravindran et al.40 N/A N2A vector control This paper N/A N2A CARP2 WT untagged This paper N/A Oligonucleotides CARP2 specific siRNA Eurogentec 50 UGACAUCUCUACCGAAAUG 30 Recombinant DNA mCherry-Golgin45 Tiwari et al.38 N/A GFP-Golgin45 Tiwari et al.38 N/A GRASP65-GFP Tang et al.65 N/A GFP-Golgin 97 Hsu et al.19 N/A (Continued on next page) 18 Cell Reports 43, 114896, November 26, 2024

    Membrane:

    Article Title: EGF-mediated Golgi dynamics and cell migration require CARP2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GM130 Sigma Cat# G7295; RRID AB_532244 Anti-GFP Takara Cat# 632375; RRID:AB_2756343 Anti-Tubulin Sigma Cat# T6199 RRID:AB_477583 Anti-Actin Cell Signaling Technology Cat# 4967 RRID:AB_330288 Anti-GAPDH Abgenex Cat# 10-10011 Anti-Flag Sigma Cat# F3165-1MG; RRID:AB_259529 Anti-HA Sigma Cat# H6908 Anti-V5 Thermo Fisher Scientific Cat# R961-25, RRID:AB_2556565 Anti-BLZF1 Thermo Fisher Scientific Cat# MA5-27126, RRID:AB_2723990 Anti-GST Sigma Cat# G7781-25UL Anti-GORASP1 Elabscience Cat# E-AB-68390 Anti-GORASP2 Elabscience Cat# E-AB-63374 Anti-GOLGA2 Elabscience Cat# E-AB-11278 Anti-CARP2 Ravindran et al.40 N/A Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 61–6520, RRID:AB_2533933 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 65–6120, RRID:AB_2533967 Peroxidase AffiniPureTM Goat Anti-mouse IgG, light chain specific Jackson ImmunoResearch Cat# 115-035-174 RRID:AB_2338512 Peroxidase -conjugated monoclonal Mouse Anti-rabbit IgG, light chain specific Jackson ImmunoResearch Cat# 211-032-171 RRID:AB_2339149 Chemicals, peptides, and recombinant proteins Adenosine 5’-triphosphate magnesium salt Sigma Cat# A9187 Dulbecco’s modified Eagle’s medium Hyclone GE Cat# SH30243.01 Fetal Bovine Serum Gibco-Thermo Fisher Scientific Cat# 10270-106 Penicillin-streptomycin antibiotic solution HiMedia Cat# A001A Lipofectamine 3000 Invitrogen Cat# L3000-015 Lipofectamine RNAi Max Invitrogen Cat# 13778150 Epidermal Growth factor Sigma Cat# E9644 MitomycinC Sigma Cat# M4287 Nile red Sigma Cat# 72485 Albumin from bovine serum Sigma Cat# A9418 Acrylamide/Bis 29:1 (30%) Biorad Cat# 1610156 Bafilomycin A1 Merck Cat# 19-148 Biotin Sigma Cat# B4501-100MG 2-mercaptoethanol Sigma Cat# M6250 bromophenol blue HiMedia Cat # MB123 DAPI Sigma Cat# D8417 EGTA Sigma Cat# 03777-50G EDTA Sigma Cat# E5134-500G Glycerol Sigma Cat #G9012 Glutathione 4 Fast Flow GE Heathcare Cat# 17513201 Glass bottom dish Cellvis Plates Cat# D35-20-1.5-N Lysozyme Sigma Cat# 62970 Hoechst Stain Invitrogen Cat# 33342 (Continued on next page) Cell Reports 43, 114896, November 26, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER MG132 solution Sigma Cat# M7449-1ML MitoTracker Red CMXRos Invitrogen Cat# M7512 Ni-NTA His BIND resin Novagen Cat# 70666-4 Opti-MEM Reduced Serum Medium Gibco-Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Sigma Cat# 441244 Phosphatase inhibitor 2 Sigma Cat# P5726-1ML Phosphatase inhibitor 3 Sigma Cat# P0044-1ML Protease inhibitor cocktail Sigma Cat# P8340-1ML Protein G agarose beads Santacruz Cat# sc-2002 Protein A agarose beads Santacruz Cat# sc-2001 Prolong Gold Antifade reagent Invitrogen Cat# P36930 PVDF membrane Merck Cat# IPVH00010 Skim milk powder Himedia Cat# GRM1254-500G Sodium chloride Sigma Cat# S9888-5KG Sodium deoxycholate Sigma Cat# D6750-25G Sodium dodecyl sulfate Sigma Cat# L3771-1KG Sodium hydroxide Sigma Cat# 1.06498 Triton X-100 Sigma Cat# X100-5ML Trizma base Sigma Cat# T6066-5KG TRYPLE EXPRESS Invitrogen Cat# 12604021 Tween 20 Sigma Cat# P2287-500ML Immobilon Western Chemiluminescent HRP Substrate Merck Cat# WBKLS0500 Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1) R&D Systems Cat# E304 Recombinant Human UbcH5a R&D Systems Cat# E2-616 Experimental models: Cell lines HEK 293T ATCC N/A HEK293T vector control This paper N/A HEK293T CARP2 WT untagged This paper N/A HEK293T mRFP alone This paper N/A HEK293T CARP2WT-mRFP This paper N/A HeLa ATCC N/A .. N2A ATCC N/A A549 vector control Ravindran et al.40 N/A A549 CARP2 WT untagged Ravindran et al.40 N/A A549 CARP2 H333A untagged Ravindran et al.40 N/A A549 CARP2 C5A/C6A untagged Ravindran et al.40 N/A A549 CARP2 KO Ravindran et al.40 N/A A549 CARP2 KO Vector control Ravindran et al.40 N/A A549 CARP2 KO CARP2WT untagged Ravindran et al.40 N/A N2A vector control This paper N/A N2A CARP2 WT untagged This paper N/A Oligonucleotides CARP2 specific siRNA Eurogentec 50 UGACAUCUCUACCGAAAUG 30 Recombinant DNA mCherry-Golgin45 Tiwari et al.38 N/A GFP-Golgin45 Tiwari et al.38 N/A GRASP65-GFP Tang et al.65 N/A GFP-Golgin 97 Hsu et al.19 N/A (Continued on next page) 18 Cell Reports 43, 114896, November 26, 2024

    Western Blot:

    Article Title: EGF-mediated Golgi dynamics and cell migration require CARP2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GM130 Sigma Cat# G7295; RRID AB_532244 Anti-GFP Takara Cat# 632375; RRID:AB_2756343 Anti-Tubulin Sigma Cat# T6199 RRID:AB_477583 Anti-Actin Cell Signaling Technology Cat# 4967 RRID:AB_330288 Anti-GAPDH Abgenex Cat# 10-10011 Anti-Flag Sigma Cat# F3165-1MG; RRID:AB_259529 Anti-HA Sigma Cat# H6908 Anti-V5 Thermo Fisher Scientific Cat# R961-25, RRID:AB_2556565 Anti-BLZF1 Thermo Fisher Scientific Cat# MA5-27126, RRID:AB_2723990 Anti-GST Sigma Cat# G7781-25UL Anti-GORASP1 Elabscience Cat# E-AB-68390 Anti-GORASP2 Elabscience Cat# E-AB-63374 Anti-GOLGA2 Elabscience Cat# E-AB-11278 Anti-CARP2 Ravindran et al.40 N/A Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 61–6520, RRID:AB_2533933 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 65–6120, RRID:AB_2533967 Peroxidase AffiniPureTM Goat Anti-mouse IgG, light chain specific Jackson ImmunoResearch Cat# 115-035-174 RRID:AB_2338512 Peroxidase -conjugated monoclonal Mouse Anti-rabbit IgG, light chain specific Jackson ImmunoResearch Cat# 211-032-171 RRID:AB_2339149 Chemicals, peptides, and recombinant proteins Adenosine 5’-triphosphate magnesium salt Sigma Cat# A9187 Dulbecco’s modified Eagle’s medium Hyclone GE Cat# SH30243.01 Fetal Bovine Serum Gibco-Thermo Fisher Scientific Cat# 10270-106 Penicillin-streptomycin antibiotic solution HiMedia Cat# A001A Lipofectamine 3000 Invitrogen Cat# L3000-015 Lipofectamine RNAi Max Invitrogen Cat# 13778150 Epidermal Growth factor Sigma Cat# E9644 MitomycinC Sigma Cat# M4287 Nile red Sigma Cat# 72485 Albumin from bovine serum Sigma Cat# A9418 Acrylamide/Bis 29:1 (30%) Biorad Cat# 1610156 Bafilomycin A1 Merck Cat# 19-148 Biotin Sigma Cat# B4501-100MG 2-mercaptoethanol Sigma Cat# M6250 bromophenol blue HiMedia Cat # MB123 DAPI Sigma Cat# D8417 EGTA Sigma Cat# 03777-50G EDTA Sigma Cat# E5134-500G Glycerol Sigma Cat #G9012 Glutathione 4 Fast Flow GE Heathcare Cat# 17513201 Glass bottom dish Cellvis Plates Cat# D35-20-1.5-N Lysozyme Sigma Cat# 62970 Hoechst Stain Invitrogen Cat# 33342 (Continued on next page) Cell Reports 43, 114896, November 26, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER MG132 solution Sigma Cat# M7449-1ML MitoTracker Red CMXRos Invitrogen Cat# M7512 Ni-NTA His BIND resin Novagen Cat# 70666-4 Opti-MEM Reduced Serum Medium Gibco-Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Sigma Cat# 441244 Phosphatase inhibitor 2 Sigma Cat# P5726-1ML Phosphatase inhibitor 3 Sigma Cat# P0044-1ML Protease inhibitor cocktail Sigma Cat# P8340-1ML Protein G agarose beads Santacruz Cat# sc-2002 Protein A agarose beads Santacruz Cat# sc-2001 Prolong Gold Antifade reagent Invitrogen Cat# P36930 PVDF membrane Merck Cat# IPVH00010 Skim milk powder Himedia Cat# GRM1254-500G Sodium chloride Sigma Cat# S9888-5KG Sodium deoxycholate Sigma Cat# D6750-25G Sodium dodecyl sulfate Sigma Cat# L3771-1KG Sodium hydroxide Sigma Cat# 1.06498 Triton X-100 Sigma Cat# X100-5ML Trizma base Sigma Cat# T6066-5KG TRYPLE EXPRESS Invitrogen Cat# 12604021 Tween 20 Sigma Cat# P2287-500ML Immobilon Western Chemiluminescent HRP Substrate Merck Cat# WBKLS0500 Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1) R&D Systems Cat# E304 Recombinant Human UbcH5a R&D Systems Cat# E2-616 Experimental models: Cell lines HEK 293T ATCC N/A HEK293T vector control This paper N/A HEK293T CARP2 WT untagged This paper N/A HEK293T mRFP alone This paper N/A HEK293T CARP2WT-mRFP This paper N/A HeLa ATCC N/A .. N2A ATCC N/A A549 vector control Ravindran et al.40 N/A A549 CARP2 WT untagged Ravindran et al.40 N/A A549 CARP2 H333A untagged Ravindran et al.40 N/A A549 CARP2 C5A/C6A untagged Ravindran et al.40 N/A A549 CARP2 KO Ravindran et al.40 N/A A549 CARP2 KO Vector control Ravindran et al.40 N/A A549 CARP2 KO CARP2WT untagged Ravindran et al.40 N/A N2A vector control This paper N/A N2A CARP2 WT untagged This paper N/A Oligonucleotides CARP2 specific siRNA Eurogentec 50 UGACAUCUCUACCGAAAUG 30 Recombinant DNA mCherry-Golgin45 Tiwari et al.38 N/A GFP-Golgin45 Tiwari et al.38 N/A GRASP65-GFP Tang et al.65 N/A GFP-Golgin 97 Hsu et al.19 N/A (Continued on next page) 18 Cell Reports 43, 114896, November 26, 2024

    Recombinant:

    Article Title: EGF-mediated Golgi dynamics and cell migration require CARP2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GM130 Sigma Cat# G7295; RRID AB_532244 Anti-GFP Takara Cat# 632375; RRID:AB_2756343 Anti-Tubulin Sigma Cat# T6199 RRID:AB_477583 Anti-Actin Cell Signaling Technology Cat# 4967 RRID:AB_330288 Anti-GAPDH Abgenex Cat# 10-10011 Anti-Flag Sigma Cat# F3165-1MG; RRID:AB_259529 Anti-HA Sigma Cat# H6908 Anti-V5 Thermo Fisher Scientific Cat# R961-25, RRID:AB_2556565 Anti-BLZF1 Thermo Fisher Scientific Cat# MA5-27126, RRID:AB_2723990 Anti-GST Sigma Cat# G7781-25UL Anti-GORASP1 Elabscience Cat# E-AB-68390 Anti-GORASP2 Elabscience Cat# E-AB-63374 Anti-GOLGA2 Elabscience Cat# E-AB-11278 Anti-CARP2 Ravindran et al.40 N/A Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 61–6520, RRID:AB_2533933 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 65–6120, RRID:AB_2533967 Peroxidase AffiniPureTM Goat Anti-mouse IgG, light chain specific Jackson ImmunoResearch Cat# 115-035-174 RRID:AB_2338512 Peroxidase -conjugated monoclonal Mouse Anti-rabbit IgG, light chain specific Jackson ImmunoResearch Cat# 211-032-171 RRID:AB_2339149 Chemicals, peptides, and recombinant proteins Adenosine 5’-triphosphate magnesium salt Sigma Cat# A9187 Dulbecco’s modified Eagle’s medium Hyclone GE Cat# SH30243.01 Fetal Bovine Serum Gibco-Thermo Fisher Scientific Cat# 10270-106 Penicillin-streptomycin antibiotic solution HiMedia Cat# A001A Lipofectamine 3000 Invitrogen Cat# L3000-015 Lipofectamine RNAi Max Invitrogen Cat# 13778150 Epidermal Growth factor Sigma Cat# E9644 MitomycinC Sigma Cat# M4287 Nile red Sigma Cat# 72485 Albumin from bovine serum Sigma Cat# A9418 Acrylamide/Bis 29:1 (30%) Biorad Cat# 1610156 Bafilomycin A1 Merck Cat# 19-148 Biotin Sigma Cat# B4501-100MG 2-mercaptoethanol Sigma Cat# M6250 bromophenol blue HiMedia Cat # MB123 DAPI Sigma Cat# D8417 EGTA Sigma Cat# 03777-50G EDTA Sigma Cat# E5134-500G Glycerol Sigma Cat #G9012 Glutathione 4 Fast Flow GE Heathcare Cat# 17513201 Glass bottom dish Cellvis Plates Cat# D35-20-1.5-N Lysozyme Sigma Cat# 62970 Hoechst Stain Invitrogen Cat# 33342 (Continued on next page) Cell Reports 43, 114896, November 26, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER MG132 solution Sigma Cat# M7449-1ML MitoTracker Red CMXRos Invitrogen Cat# M7512 Ni-NTA His BIND resin Novagen Cat# 70666-4 Opti-MEM Reduced Serum Medium Gibco-Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Sigma Cat# 441244 Phosphatase inhibitor 2 Sigma Cat# P5726-1ML Phosphatase inhibitor 3 Sigma Cat# P0044-1ML Protease inhibitor cocktail Sigma Cat# P8340-1ML Protein G agarose beads Santacruz Cat# sc-2002 Protein A agarose beads Santacruz Cat# sc-2001 Prolong Gold Antifade reagent Invitrogen Cat# P36930 PVDF membrane Merck Cat# IPVH00010 Skim milk powder Himedia Cat# GRM1254-500G Sodium chloride Sigma Cat# S9888-5KG Sodium deoxycholate Sigma Cat# D6750-25G Sodium dodecyl sulfate Sigma Cat# L3771-1KG Sodium hydroxide Sigma Cat# 1.06498 Triton X-100 Sigma Cat# X100-5ML Trizma base Sigma Cat# T6066-5KG TRYPLE EXPRESS Invitrogen Cat# 12604021 Tween 20 Sigma Cat# P2287-500ML Immobilon Western Chemiluminescent HRP Substrate Merck Cat# WBKLS0500 Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1) R&D Systems Cat# E304 Recombinant Human UbcH5a R&D Systems Cat# E2-616 Experimental models: Cell lines HEK 293T ATCC N/A HEK293T vector control This paper N/A HEK293T CARP2 WT untagged This paper N/A HEK293T mRFP alone This paper N/A HEK293T CARP2WT-mRFP This paper N/A HeLa ATCC N/A .. N2A ATCC N/A A549 vector control Ravindran et al.40 N/A A549 CARP2 WT untagged Ravindran et al.40 N/A A549 CARP2 H333A untagged Ravindran et al.40 N/A A549 CARP2 C5A/C6A untagged Ravindran et al.40 N/A A549 CARP2 KO Ravindran et al.40 N/A A549 CARP2 KO Vector control Ravindran et al.40 N/A A549 CARP2 KO CARP2WT untagged Ravindran et al.40 N/A N2A vector control This paper N/A N2A CARP2 WT untagged This paper N/A Oligonucleotides CARP2 specific siRNA Eurogentec 50 UGACAUCUCUACCGAAAUG 30 Recombinant DNA mCherry-Golgin45 Tiwari et al.38 N/A GFP-Golgin45 Tiwari et al.38 N/A GRASP65-GFP Tang et al.65 N/A GFP-Golgin 97 Hsu et al.19 N/A (Continued on next page) 18 Cell Reports 43, 114896, November 26, 2024

    Plasmid Preparation:

    Article Title: EGF-mediated Golgi dynamics and cell migration require CARP2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GM130 Sigma Cat# G7295; RRID AB_532244 Anti-GFP Takara Cat# 632375; RRID:AB_2756343 Anti-Tubulin Sigma Cat# T6199 RRID:AB_477583 Anti-Actin Cell Signaling Technology Cat# 4967 RRID:AB_330288 Anti-GAPDH Abgenex Cat# 10-10011 Anti-Flag Sigma Cat# F3165-1MG; RRID:AB_259529 Anti-HA Sigma Cat# H6908 Anti-V5 Thermo Fisher Scientific Cat# R961-25, RRID:AB_2556565 Anti-BLZF1 Thermo Fisher Scientific Cat# MA5-27126, RRID:AB_2723990 Anti-GST Sigma Cat# G7781-25UL Anti-GORASP1 Elabscience Cat# E-AB-68390 Anti-GORASP2 Elabscience Cat# E-AB-63374 Anti-GOLGA2 Elabscience Cat# E-AB-11278 Anti-CARP2 Ravindran et al.40 N/A Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 61–6520, RRID:AB_2533933 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 65–6120, RRID:AB_2533967 Peroxidase AffiniPureTM Goat Anti-mouse IgG, light chain specific Jackson ImmunoResearch Cat# 115-035-174 RRID:AB_2338512 Peroxidase -conjugated monoclonal Mouse Anti-rabbit IgG, light chain specific Jackson ImmunoResearch Cat# 211-032-171 RRID:AB_2339149 Chemicals, peptides, and recombinant proteins Adenosine 5’-triphosphate magnesium salt Sigma Cat# A9187 Dulbecco’s modified Eagle’s medium Hyclone GE Cat# SH30243.01 Fetal Bovine Serum Gibco-Thermo Fisher Scientific Cat# 10270-106 Penicillin-streptomycin antibiotic solution HiMedia Cat# A001A Lipofectamine 3000 Invitrogen Cat# L3000-015 Lipofectamine RNAi Max Invitrogen Cat# 13778150 Epidermal Growth factor Sigma Cat# E9644 MitomycinC Sigma Cat# M4287 Nile red Sigma Cat# 72485 Albumin from bovine serum Sigma Cat# A9418 Acrylamide/Bis 29:1 (30%) Biorad Cat# 1610156 Bafilomycin A1 Merck Cat# 19-148 Biotin Sigma Cat# B4501-100MG 2-mercaptoethanol Sigma Cat# M6250 bromophenol blue HiMedia Cat # MB123 DAPI Sigma Cat# D8417 EGTA Sigma Cat# 03777-50G EDTA Sigma Cat# E5134-500G Glycerol Sigma Cat #G9012 Glutathione 4 Fast Flow GE Heathcare Cat# 17513201 Glass bottom dish Cellvis Plates Cat# D35-20-1.5-N Lysozyme Sigma Cat# 62970 Hoechst Stain Invitrogen Cat# 33342 (Continued on next page) Cell Reports 43, 114896, November 26, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER MG132 solution Sigma Cat# M7449-1ML MitoTracker Red CMXRos Invitrogen Cat# M7512 Ni-NTA His BIND resin Novagen Cat# 70666-4 Opti-MEM Reduced Serum Medium Gibco-Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Sigma Cat# 441244 Phosphatase inhibitor 2 Sigma Cat# P5726-1ML Phosphatase inhibitor 3 Sigma Cat# P0044-1ML Protease inhibitor cocktail Sigma Cat# P8340-1ML Protein G agarose beads Santacruz Cat# sc-2002 Protein A agarose beads Santacruz Cat# sc-2001 Prolong Gold Antifade reagent Invitrogen Cat# P36930 PVDF membrane Merck Cat# IPVH00010 Skim milk powder Himedia Cat# GRM1254-500G Sodium chloride Sigma Cat# S9888-5KG Sodium deoxycholate Sigma Cat# D6750-25G Sodium dodecyl sulfate Sigma Cat# L3771-1KG Sodium hydroxide Sigma Cat# 1.06498 Triton X-100 Sigma Cat# X100-5ML Trizma base Sigma Cat# T6066-5KG TRYPLE EXPRESS Invitrogen Cat# 12604021 Tween 20 Sigma Cat# P2287-500ML Immobilon Western Chemiluminescent HRP Substrate Merck Cat# WBKLS0500 Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1) R&D Systems Cat# E304 Recombinant Human UbcH5a R&D Systems Cat# E2-616 Experimental models: Cell lines HEK 293T ATCC N/A HEK293T vector control This paper N/A HEK293T CARP2 WT untagged This paper N/A HEK293T mRFP alone This paper N/A HEK293T CARP2WT-mRFP This paper N/A HeLa ATCC N/A .. N2A ATCC N/A A549 vector control Ravindran et al.40 N/A A549 CARP2 WT untagged Ravindran et al.40 N/A A549 CARP2 H333A untagged Ravindran et al.40 N/A A549 CARP2 C5A/C6A untagged Ravindran et al.40 N/A A549 CARP2 KO Ravindran et al.40 N/A A549 CARP2 KO Vector control Ravindran et al.40 N/A A549 CARP2 KO CARP2WT untagged Ravindran et al.40 N/A N2A vector control This paper N/A N2A CARP2 WT untagged This paper N/A Oligonucleotides CARP2 specific siRNA Eurogentec 50 UGACAUCUCUACCGAAAUG 30 Recombinant DNA mCherry-Golgin45 Tiwari et al.38 N/A GFP-Golgin45 Tiwari et al.38 N/A GRASP65-GFP Tang et al.65 N/A GFP-Golgin 97 Hsu et al.19 N/A (Continued on next page) 18 Cell Reports 43, 114896, November 26, 2024

    Control:

    Article Title: EGF-mediated Golgi dynamics and cell migration require CARP2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GM130 Sigma Cat# G7295; RRID AB_532244 Anti-GFP Takara Cat# 632375; RRID:AB_2756343 Anti-Tubulin Sigma Cat# T6199 RRID:AB_477583 Anti-Actin Cell Signaling Technology Cat# 4967 RRID:AB_330288 Anti-GAPDH Abgenex Cat# 10-10011 Anti-Flag Sigma Cat# F3165-1MG; RRID:AB_259529 Anti-HA Sigma Cat# H6908 Anti-V5 Thermo Fisher Scientific Cat# R961-25, RRID:AB_2556565 Anti-BLZF1 Thermo Fisher Scientific Cat# MA5-27126, RRID:AB_2723990 Anti-GST Sigma Cat# G7781-25UL Anti-GORASP1 Elabscience Cat# E-AB-68390 Anti-GORASP2 Elabscience Cat# E-AB-63374 Anti-GOLGA2 Elabscience Cat# E-AB-11278 Anti-CARP2 Ravindran et al.40 N/A Rabbit anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 61–6520, RRID:AB_2533933 Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 65–6120, RRID:AB_2533967 Peroxidase AffiniPureTM Goat Anti-mouse IgG, light chain specific Jackson ImmunoResearch Cat# 115-035-174 RRID:AB_2338512 Peroxidase -conjugated monoclonal Mouse Anti-rabbit IgG, light chain specific Jackson ImmunoResearch Cat# 211-032-171 RRID:AB_2339149 Chemicals, peptides, and recombinant proteins Adenosine 5’-triphosphate magnesium salt Sigma Cat# A9187 Dulbecco’s modified Eagle’s medium Hyclone GE Cat# SH30243.01 Fetal Bovine Serum Gibco-Thermo Fisher Scientific Cat# 10270-106 Penicillin-streptomycin antibiotic solution HiMedia Cat# A001A Lipofectamine 3000 Invitrogen Cat# L3000-015 Lipofectamine RNAi Max Invitrogen Cat# 13778150 Epidermal Growth factor Sigma Cat# E9644 MitomycinC Sigma Cat# M4287 Nile red Sigma Cat# 72485 Albumin from bovine serum Sigma Cat# A9418 Acrylamide/Bis 29:1 (30%) Biorad Cat# 1610156 Bafilomycin A1 Merck Cat# 19-148 Biotin Sigma Cat# B4501-100MG 2-mercaptoethanol Sigma Cat# M6250 bromophenol blue HiMedia Cat # MB123 DAPI Sigma Cat# D8417 EGTA Sigma Cat# 03777-50G EDTA Sigma Cat# E5134-500G Glycerol Sigma Cat #G9012 Glutathione 4 Fast Flow GE Heathcare Cat# 17513201 Glass bottom dish Cellvis Plates Cat# D35-20-1.5-N Lysozyme Sigma Cat# 62970 Hoechst Stain Invitrogen Cat# 33342 (Continued on next page) Cell Reports 43, 114896, November 26, 2024 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER MG132 solution Sigma Cat# M7449-1ML MitoTracker Red CMXRos Invitrogen Cat# M7512 Ni-NTA His BIND resin Novagen Cat# 70666-4 Opti-MEM Reduced Serum Medium Gibco-Thermo Fisher Scientific Cat# 31985070 Paraformaldehyde Sigma Cat# 441244 Phosphatase inhibitor 2 Sigma Cat# P5726-1ML Phosphatase inhibitor 3 Sigma Cat# P0044-1ML Protease inhibitor cocktail Sigma Cat# P8340-1ML Protein G agarose beads Santacruz Cat# sc-2002 Protein A agarose beads Santacruz Cat# sc-2001 Prolong Gold Antifade reagent Invitrogen Cat# P36930 PVDF membrane Merck Cat# IPVH00010 Skim milk powder Himedia Cat# GRM1254-500G Sodium chloride Sigma Cat# S9888-5KG Sodium deoxycholate Sigma Cat# D6750-25G Sodium dodecyl sulfate Sigma Cat# L3771-1KG Sodium hydroxide Sigma Cat# 1.06498 Triton X-100 Sigma Cat# X100-5ML Trizma base Sigma Cat# T6066-5KG TRYPLE EXPRESS Invitrogen Cat# 12604021 Tween 20 Sigma Cat# P2287-500ML Immobilon Western Chemiluminescent HRP Substrate Merck Cat# WBKLS0500 Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1) R&D Systems Cat# E304 Recombinant Human UbcH5a R&D Systems Cat# E2-616 Experimental models: Cell lines HEK 293T ATCC N/A HEK293T vector control This paper N/A HEK293T CARP2 WT untagged This paper N/A HEK293T mRFP alone This paper N/A HEK293T CARP2WT-mRFP This paper N/A HeLa ATCC N/A .. N2A ATCC N/A A549 vector control Ravindran et al.40 N/A A549 CARP2 WT untagged Ravindran et al.40 N/A A549 CARP2 H333A untagged Ravindran et al.40 N/A A549 CARP2 C5A/C6A untagged Ravindran et al.40 N/A A549 CARP2 KO Ravindran et al.40 N/A A549 CARP2 KO Vector control Ravindran et al.40 N/A A549 CARP2 KO CARP2WT untagged Ravindran et al.40 N/A N2A vector control This paper N/A N2A CARP2 WT untagged This paper N/A Oligonucleotides CARP2 specific siRNA Eurogentec 50 UGACAUCUCUACCGAAAUG 30 Recombinant DNA mCherry-Golgin45 Tiwari et al.38 N/A GFP-Golgin45 Tiwari et al.38 N/A GRASP65-GFP Tang et al.65 N/A GFP-Golgin 97 Hsu et al.19 N/A (Continued on next page) 18 Cell Reports 43, 114896, November 26, 2024



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